Want To Download, "Automated PTMScan® immunoaffinity enrichment for the capture of KGG modified peptides from complex mixtures"?

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Abstract

Recent advances in mass spectrometry instrumentation and sample handling have enabled researchers to routinely perform global profiling of many protein post-translational modifications, expanding our knowledge of biological pathways. One key to the success of these experiments is the effective selective enrichment of the modified peptides from complex mixtures before introduction to the mass spectrometer, often via immunoaffinity purification using antibodies that are directed against the PTM of interest. Here, using the ubiquitin remnant motif (KGG) antibody as a model, we expand on the PTMScan® immunoaffinity enrichment protocol by coupling it to the Phynexus MEA robot, developing a robust automated platform that enables the concurrent processing of up to twelve samples with limited manual sample handling. We demonstrate the utility of the automated system in the identification of thousands of KGG peptides from complex biological samples.