PhyTip® Columns Containing CaptureSelect® Resins for Purification of Antibodies and Antibody Fragments

Introduction

The Biotage PhyTip®  columns are the proven technology of choice for the purification of recombinant proteins and antibodies when requiring robust high quality reproducible separations from small-volume samples while maintaining high protein activity. The innovative methods developed for processing PhyTip columns result in equilibrium binding and elution and have virtually no dead volume allowing for efficient capture and the highest concentration of eluted active proteins possible with results that are scalable to manufacturing volumes (Figure 1). The columns are supported by many liquid handling systems for ease of integration and high throughput.

 

Biotage is excited to offer PhyTip columns containing the CaptureSelect® resins from BAC BV. As the new spectrum of antibody formats evolves the current affinity ligands cannot always provide the binding and elution specificity demanded. CaptureSelect products are affinity ligands created by a proprietary technology based on Camelid derived single domain antibody fragments. These products possess a combination of unique properties such as stability, affinity, and selectivity that provide competitive benefits in terms of reduced cost of purification, higher quality product, reduced development and increased flexibility in the purification process to eliminate uncertainty, streamline protocols and ensure confidence that the final product is the intended antibody species. This is achieved through specific affinities to novel antibody domains.