Optimization Strategies for High Performance Purification and Analysis of Recombinant Proteins with Micro Volume PhyTip® Columns and Caliper Life Sciences Automation

In the post genomic era of drug discovery, the need to prepare and analyze large numbers of recombinant proteins and antibodies has increased significantly, which in turn is driving the process of efficient expression and purification to higher throughput and lower volume technologies. PhyTip® columns are unique separation columns designed to facilitate the purification and evaluation of recombinant proteins and antibodies from relatively low volumes of starting material in a high throughput format. The ability to pack any separation resin such as ion exchange or affinity resins in the PhyTip column and process from any sample type with automation such as the liquid handling systems provided by Caliper Life Sciences allows for efficient purification and screening of resins, early stage constructs, and purification conditions in a completely automated process. Subsequent data analysis using Caliper Life Sciences LabChip GXII eliminates time consuming assays and gels and provides a total solution for bioprocess development applications and expedites the drug discovery process. Purification with PhyTip columns is a simple process that includes capturing the protein of interest on the resin, purifying the protein by washing away the non specific binding products and finally eluting with a small volume of enrichment buffer as low as 10 μL. The flexibility of using various resins, buffer and elution conditions on liquid handling systems with the Labchip GXII system in a single experiment allows for unprecedented amounts of scalable information for complex data generation and analysis never before achievable with a single automated high throughput method.