Now that you have your purified plasmid, you will need to get an idea of its quality. Transfection efficiency is highly influenced by the quality of the plasmid, so it’s important to get a full picture. There are a few factors to plasmid quality, and you will want to look at all of them to make sure your samples will be successful in your downstream processing. Here, I will explain how to measure the absorbance ratios of your sample, successfully read an agarose gel, and the importance of low endotoxin values.

Successful transient transfection relies on many factors, starting with a successful plasmid prep. To ensure high transfection efficiency, resulting DNA from your plasmid preps must be transfection grade. But what does transfection grade really mean?

One of the biggest challenges with automated plasmid purification is with culture growth conditions, leading to low yielding cultures. Many times, culture growth is unpredictable and hard to perfect. Large volume culture growth is an additional struggle. But it’s important to optimize your culture growth, because when your culture growth conditions are reliable, your downstream processing will be more successful and higher yielding. Here are a couple recommendations for successful large scale culture growth.