Plasmids are one of the cornerstones of modern biology, providing the foundation for several areas of important research including DNA vaccines, mRNA vaccines, viral vectors, and monoclonal antibodies. This small, circular DNA is found in bacterial cells and is used widely as a vector for specific DNA fragments across multiple fields, including molecular biology, biochemistry, biotechnology, and cell biology. Plasmid purification, therefore, has a wide range of applications and its importance is reflected in recent scientific triumphs including the development of COVID-19 vaccines and advancements in gene and cell therapies.