Gene therapy has had its share of setbacks, but finally gene therapies are proving successful at treating diseases like neuromuscular disorders, cancer and blindness. Adeno-associated virus (AAV) has emerged as a leading gene delivery vehicle because it is efficient and has demonstrated safety in humans.
Plasmids are one of the cornerstones of modern biology, providing the foundation for several areas of important research including DNA vaccines, mRNA vaccines, viral vectors, and monoclonal antibodies. This small, circular DNA is found in bacterial cells and is used widely as a vector for specific DNA fragments across multiple fields, including molecular biology, biochemistry, biotechnology, and cell biology. Plasmid purification, therefore, has a wide range of applications and its importance is reflected in recent scientific triumphs including the development of COVID-19 vaccines and advancements in gene and cell therapies.