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      How to check the quality of plasmid DNA

      Jul 20, 2022 12:27:39 AM / by Megan Gallagher posted in Maxiprep, Megaprep, Gigaprep, Plasmid, Plasmid Purification, transfection, transient transfection, transfection grade plasmid DNA, Endotoxin, DNA, Supercoiled, Circular DNA, Transfection-grade

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      Now that you have your purified plasmid, you will need to get an idea of its quality. Transfection efficiency is highly influenced by the quality of the plasmid, so it’s important to get a full picture. There are a few factors to plasmid quality, and you will want to look at all of them to make sure your samples will be successful in your downstream processing. Here, I will explain how to measure the absorbance ratios of your sample, successfully read an agarose gel, and the importance of low endotoxin values.

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      Transfection Efficiency: What Makes Plasmid DNA Transfection Grade

      Apr 28, 2022 2:30:00 PM / by Megan Gallagher posted in Maxiprep, Megaprep, Gigaprep, Plasmid, Plasmid Purification, transfection, transient transfection, transfection grade plasmid DNA, Endotoxin, DNA, Supercoiled, Circular DNA, Transfection-grade

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      Successful transient transfection relies on many factors, starting with a successful plasmid prep. To ensure high transfection efficiency, resulting DNA from your plasmid preps must be transfection grade. But what does transfection grade really mean?

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