Welcome to the Biotage Biomolecules Blogs.

      How to Select Chromatography Media for the Purification of Antibodies

      Oct 28, 2020 7:00:00 AM / by Elinor Meiby posted in Automation, PhyTip, IgG purification

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      Purification of immunoglobulins is essential for the development of new biopharmaceuticals and affinity chromatography is a popular method for this application. One approach to purification of immunoglobulins it to employ histidine tags, which make binding to metal ions stronger and therefore chromatographic purification more efficient. However, when working with antibodies that are expressed without such a tag, affinity medias with immobilized ligands with binding affinity to specific regions of the antibody can be used for chromatographic purification.

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      Technologies for Automated High-Throughput Protein Isolation

      Oct 7, 2020 7:00:00 AM / by Elinor Meiby posted in Automation, PhyTip, purification, IMAC, IgG purification

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      High-throughput methods for protein purification and production are becoming more common in the biopharmaceutical and biotech industry. Advancements have occurred in high-throughput expression and cloning technologies. Automated protein purification methods have led to increased productivity and reproducibility. However,  what technologies are available for automated high-throughput protein extraction? In the book “High-throughput protein production and purification” (link below)various authors have contributed with their protocols for their expression pipelines. This blog posts provides an overview of the automated methods for protein purification that were described here – what applications they were used for, which principles the methods are based on and how they are automated. 

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      High Performance Direct and Indirect Immunoprecipitation

      Sep 23, 2020 9:15:00 AM / by Elinor Meiby posted in Automation, Isolera, Protein G

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      Immunoprecipitation is a well-known method in biomedical research for protein isolation. This method is useful to be able to study a protein’s physiochemical properties, post-translational modifications or expressions in various tissues or cell lines. In immunoprecipitation, the protein is captured on a solid support with an immobilized antibody that binds to the protein in question.

      Immunoprecipitation is usually carried out in small tubes by the use of magnetic beads or loose affinity resin as solid support.

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      Protein Expression Analysis - Fast yet Flexible

      Dec 16, 2019 11:38:53 AM / by Elinor Meiby posted in Customer case, Automation, PhyTip

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      For anyone looking to automate their protein expression platform, the book “High-Throughput Protein Production and Purification” that was published in July 2019 (ISBN 978-1-4939-9624-7) is an excellent read. 

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      Perfusion vs. Fed-batch Bioreactors - are there any Differences in Protein Quality?

      Dec 16, 2019 11:36:35 AM / by Elinor Meiby posted in Automation, PhyTip

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      According to a relatively newly published journal article (Bielser et al. Aug 20 2019, J Biotech), there has been a renewed interest in the science community in perfusion cell culture technologies for continuous and efficient production of therapeutic recombinant proteins. 

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