Purification of immunoglobulins is essential for the development of new biopharmaceuticals and affinity chromatography is a popular method for this application. One approach to purification of immunoglobulins it to employ histidine tags, which make binding to metal ions stronger and therefore chromatographic purification more efficient. However, when working with antibodies that are expressed without such a tag, affinity medias with immobilized ligands with binding affinity to specific regions of the antibody can be used for chromatographic purification.
How to Select Chromatography Media for the Purification of Antibodies
Oct 28, 2020 7:00:00 AM / by Elinor Meiby posted in Automation, PhyTip, IgG purification
Technologies for Automated High-Throughput Protein Isolation
Oct 7, 2020 7:00:00 AM / by Elinor Meiby posted in Automation, PhyTip, purification, IMAC, IgG purification
High-throughput methods for protein purification and production are becoming more common in the biopharmaceutical and biotech industry. Advancements have occurred in high-throughput expression and cloning technologies. Automated protein purification methods have led to increased productivity and reproducibility. However, what technologies are available for automated high-throughput protein extraction? In the book “High-throughput protein production and purification” (link below), various authors have contributed with their protocols for their expression pipelines. This blog posts provides an overview of the automated methods for protein purification that were described here – what applications they were used for, which principles the methods are based on and how they are automated.
Dual Flow Chromatography - Protein Purification in a Pipette Tip
Jun 22, 2020 5:20:29 PM / by Elinor Meiby posted in PhyTip, Protein G
Dual flow chromatography (DFC) is the unique process of performing chromatography separations in a column bed loosely packed at the end of a pipette tip, retained by a minimally absorbing, proprietary mesh – a PhyTip®. Why? To enable automated sample purification on automated liquid handling systems while obtaining high sample recovery and purity.
Protein Expression Analysis - Fast yet Flexible
Dec 16, 2019 11:38:53 AM / by Elinor Meiby posted in Customer case, Automation, PhyTip
For anyone looking to automate their protein expression platform, the book “High-Throughput Protein Production and Purification” that was published in July 2019 (ISBN 978-1-4939-9624-7) is an excellent read.
Perfusion vs. Fed-batch Bioreactors - are there any Differences in Protein Quality?
Dec 16, 2019 11:36:35 AM / by Elinor Meiby posted in Automation, PhyTip
According to a relatively newly published journal article (Bielser et al. Aug 20 2019, J Biotech), there has been a renewed interest in the science community in perfusion cell culture technologies for continuous and efficient production of therapeutic recombinant proteins.