Reversed-phase chromatography is an essential step in any drug discovery workflow, particularly as a desalting step prior to MS/MALDI analysis. One of the first choices you must make when you design your purification protocol is resin selection, and there are several options to choose from depending on your sample type and downstream application. One important variable to consider among different resin types is pore size.
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Since the therapeutic use of antibodies for cancer continues to grow since Kohler and Milstein’s work on the hybridoma technology, the need for high throughput antibody purification has become a staple in many areas of drug discovery and development process.
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