High-throughput methods for protein purification and production are becoming more common in the biopharmaceutical and biotech industry. Advancements have occurred in high-throughput expression and cloning technologies. Automated protein purification methods have led to increased productivity and reproducibility. However, what technologies are available for automated high-throughput protein extraction? In the book “High-throughput protein production and purification” (link below), various authors have contributed with their protocols for their expression pipelines. This blog posts provides an overview of the automated methods for protein purification that were described here – what applications they were used for, which principles the methods are based on and how they are automated.