Welcome to the Biotage Biomolecules Blogs.

      High Performance Direct and Indirect Immunoprecipitation

      Sep 23, 2020 9:15:00 AM / by Elinor Meiby posted in Automation, Isolera, Protein G

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      Immunoprecipitation is a well-known method in biomedical research for protein isolation. This method is useful to be able to study a protein’s physiochemical properties, post-translational modifications or expressions in various tissues or cell lines. In immunoprecipitation, the protein is captured on a solid support with an immobilized antibody that binds to the protein in question.

      Immunoprecipitation is usually carried out in small tubes by the use of magnetic beads or loose affinity resin as solid support.

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      Dual Flow Chromatography - Protein Purification in a Pipette Tip

      Jun 22, 2020 5:20:29 PM / by Elinor Meiby posted in PhyTip, Protein G

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      Dual flow chromatography (DFC) is the unique process of performing chromatography separations in a column bed loosely packed at the end of a pipette tip, retained by a minimally absorbing, proprietary mesh – a PhyTip®. Why? To enable automated sample purification on automated liquid handling systems while obtaining high sample recovery and purity.

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      Protein Expression Analysis - Fast yet Flexible

      Dec 16, 2019 11:38:53 AM / by Elinor Meiby posted in Customer case, Automation, PhyTip

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      For anyone looking to automate their protein expression platform, the book “High-Throughput Protein Production and Purification” that was published in July 2019 (ISBN 978-1-4939-9624-7) is an excellent read. 

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      Perfusion vs. Fed-batch Bioreactors - are there any Differences in Protein Quality?

      Dec 16, 2019 11:36:35 AM / by Elinor Meiby posted in Automation, PhyTip

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      According to a relatively newly published journal article (Bielser et al. Aug 20 2019, J Biotech), there has been a renewed interest in the science community in perfusion cell culture technologies for continuous and efficient production of therapeutic recombinant proteins. 

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